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mCherry antibody

Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508
Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508
Anti-mCherry antibody used in IHC (Frozen sections) (IHC-Fr). GTX128508
Anti-mCherry antibody used in IHC (Frozen sections) (IHC-Fr). GTX128508
Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508

Cat. No. GTX128508

Reference 54
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm, IP, PLA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all mCherry products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2721247

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508

GTX128508 IHC-Wm Image

mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.
Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

GTX128508 ICC/IF Image

mCherry antibody detects mCherry protein at mCherry fusion protein by immunofluorescent analysis.
Sample: mCherry fusion protein-transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 30 min, then Triton X-100 for 1 min.
Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
Red: mCherry is expressed in the transfected cell . Blue: Hoechst 33342 staining.

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody (GTX128508) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128508

GTX128508 IP Image

mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

GTX128508 ICC/IF Image

The data was published in the journal Nat Commun in 2017.PMID: 29203870

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

GTX128508 ICC/IF Image

The data was published in the journal EMBO J in 2019.PMID: 31368584

Anti-mCherry antibody used in IHC (Frozen sections) (IHC-Fr). GTX128508

GTX128508 IHC-Fr Image

The data was published in the journal Nat Commun in 2017.PMID: 28466843

Anti-mCherry antibody used in IHC (Frozen sections) (IHC-Fr). GTX128508

GTX128508 IHC-Fr Image

The data was published in the journal Nat Commun in 2017.PMID: 28466843

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

GTX128508 ICC/IF Image

The data was published in the journal Nat Commun in 2017.PMID: 29203870

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

The data was published in the journal Front Cell Dev Biol in 2020.PMID: 32457908

REFERENCE

Application Reference

SM de Leeuw et al. STAR Protoc 2023; 5 (1) : 102779 Intracellular cholesterol visualization in brain tissue using D4H
Corno A et al. EMBO J 2023; 42 (20) : e112630 A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.
Clarke JP et al. Front Mol Biosci 2023; 10 : 1178439 Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.
Yang ZH et al. BMC Med 2023; 21 (1) : 254 Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.
Longarini EJ et al. Mol Cell 2023; 83 (10) : 1743-1760.e11 Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
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Submit a Reference

REVIEW

mCherry antibody Cat. No. GTX128508

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Date : Anonymous submitted on 21-Jul-2021
Score :
Application Tested : WB
Sample : Plasma of protein X-mCherry knockin mouse
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila Brain
Blocking : 10% NGS in 0.3% PBST, 25°C, 4Hr
Fixation : 4% PFA, 4°C, overnightHr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
SM de Leeuw et al. STAR Protoc 2023; 5 (1):102779 Intracellular cholesterol visualization in brain tissue using D4H
Corno A et al. EMBO J 2023; 42 (20):e112630 A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.
Clarke JP et al. Front Mol Biosci 2023; 10:1178439 Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.
Yang ZH et al. BMC Med 2023; 21 (1):254 Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.
Longarini EJ et al. Mol Cell 2023; 83 (10):1743-1760.e11 Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Dabrowski R et al. J Cell Biol 2023; 222 (7) Parallel phospholipid transfer by Vps13 and Atg2 determines autophagosome biogenesis dynamics.
Weng M et al. J Virol 2023; 97 (3):e0154522 Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation.
Application : WB
Galer EL et al. J Neurosci 2023; 43 (3):419-432 Cervical Epidural Electrical Stimulation Increases Respiratory Activity through Somatostatin-Expressing Neurons in the Dorsal Cervical Spinal Cord in Rats.
Preston AJ et al. Cell Death Discov 2023; 9 (1):66 Elephant TP53-RETROGENE 9 induces transcription-independent apoptosis at the mitochondria.
Application : WB, ICC/IF
Buijs TJ et al. EMBO J 2023; 42 (3):e111348 STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons.
Application : ICC/IF
Feringa FM et al. J Neurosci 2023; 43 (3):347-358 An Atypical, Staged Cell Death Pathway Induced by Depletion of SNARE-Proteins MUNC18-1 or Syntaxin-1.
Application : ICC/IF
Cocozza F et al. EMBO J 2023; 42 (24):e113590 Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic?cells.
Guh CY et al. Nat Commun 2022; 13 (1):5781 XPF activates break-induced telomere synthesis.
Application : WB
Su W et al. Nat Commun 2022; 13 (1):6577 Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice.
Application : WB, IP
Fechner J et al. Int J Mol Sci 2022; 23 (20) The Binding of CSL Proteins to Either Co-Activators or Co-Repressors Protects from Proteasomal Degradation Induced by MAPK-Dependent Phosphorylation.
Rui Chen et al. Genome Med 2022; 14 (1):53 Functional genomic analysis delineates regulatory mechanisms of GWAS-identified bipolar disorder risk variants.
Application : ICC/IF
Banta KL et al. Immunity 2022; 55 (3):512-526.e9 Mechanistic convergence of the TIGIT and PD-1 inhibitory pathways necessitates co-blockade to optimize anti-tumor CD8+ T?cell responses.
Application : WB
Buser DP et al. Life Sci Alliance 2022; 5 (7) Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.
Wang L et al. Microbiol Spectr 2022;:e0186021 Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.
Application : WB
Toni Beltran et al. EMBO J 2021; 40 (5):e105564 Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors.
Application : IHC-Fr
Ahmet C Berkyurek et al. EMBO J 2021; 40 (5):e105565 The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription.
van Berkel AA et al. J Neurochem 2021; 157 (3):450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.
Chassefeyre R et al. Sci Adv 2021; 7 (52):eabg3693 Endosomal sorting drives the formation of axonal prion protein endoggresomes.
Application : WB
Nader GPF et al. Cell 2021; 184 (20):5230-5246.e22 Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion.
Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Application : IHC-Wm
Nagashima S et al. J Biol Chem 2021; 297 (1):100803 CSE1L promotes nuclear accumulation of transcriptional coactivator TAZ and enhances invasiveness of human cancer?cells.
Corrales RM et al. PLoS Pathog 2021; 17 (6):e1009666 The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host.
Suzuki S et al. Mol Cell Biochem 2021; 476 (4):1673-1690 DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.
Yuki Miura et al. Nat Biotechnol 2020; 38 (12):1421-1430 Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Wang C et al. Front Cell Dev Biol 2020; 8:329 PCDH7 Inhibits the Formation of Homotypic Cell-in-Cell Structure.
Wang LT et al. EMBO Rep 2020; 21 (2):e48795 PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition.
Melick CH et al. J Biol Chem 2020; 295 (23):8096-8105 A-kinase anchoring protein 8L interacts with mTORC1 and promotes cell growth.
Application : WB
Sim?es FC et al. Nat Commun 2020; 11 (1):600 Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair.
Application : IHC-Fr
Yang Z et al. Mol Psychiatry 2020; 25 (1):48-66 The genome-wide risk alleles for psychiatric disorders at 3p21.1 show convergent effects on mRNA expression, cognitive function, and mushroom dendritic spine.
Pan X et al. Sci Rep 2019; 9 (1):19110 FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Application : WB
Smith RJ et al. Cell Rep 2019; 28 (8):2206-2219.e8 PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.
Application : ICC/IF
Miyagawa T et al. J Cell Biol 2019; 218 (10):3355-3371 MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane.
Application : PLA
Chang CH et al. Cell Rep 2019; 28 (5):1195-1205.e3 The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice.
Application : IHC-Fr
Brouwer M et al. EMBO J 2019;:e101289 SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering.
Application : ICC/IF
Ikeda KN et al. J Biol Chem 2019; 294 (30):11486-11497 Spatial proteomics reveal that the protein phosphatase PTP1B interacts with and may modify tyrosine phosphorylation of the rhomboid protease RHBDL4.
Shahror RA et al. J Neurotrauma 2019; (Epub) Transplantation of Mesenchymal Stem Cells Overexpressing FGF21 Facilitates Cognitive Recovery and Enhances Neurogenesis in a Mouse Model of Traumatic Brain Injury.
Application : IHC-Fr
Jewell JL et al. Elife 2019; 8 GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor.
Richard J Smith et al. bioRxiv 2019; (Epub) PP1 and PP2A use opposite phospho-dependencies to control distinct processes at the kinetochore.
Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Enya S et al. Dis Model Mech 2018; 11 (8) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.
Stewart SE et al. J Cell Sci 2018; 131 (14) Transbilayer phospholipid movement facilitates the translocation of annexin across membranes.
Zhang Q et al. Cell 2018; 174 (4):870-883.e17 The Mitochondrial Unfolded Protein Response Is Mediated Cell-Non-autonomously by Retromer-Dependent Wnt Signaling.
Yao X et al. Dev Cell 2018; 45 (4):526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.
Application : IHC-Fr
Stewart SE et al. bioRxiv 2018; (Epub) Phospholipid flipping facilitates annexin translocation across membranes.
Application : WB
Taulet N et al. Nat Commun 2017; 8 (1):1928 IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Application : ICC/IF
Pfefferli C et al. Nat Commun 2017; 8:15151 The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin.
Yao X et al. Cell Res 2017; 27 (6):801-814 Homology-mediated end joining-based targeted integration using CRISPR/Cas9.
Application : IHC-Fr
Yang FM et al. Biochem J 2015; 471 (2):199-209 A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding.
Application : WB
Chou CM et al. J Biomed Sci 2015; 22:102 Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169