GeneTex

  • Country / Location Selection

United States (US)

Raf1 antibody [N1C1-2]

Overexpression
Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588
Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588
Anti-Raf1 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX111588
Anti-Raf1 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111588

Cat. No. GTX111588

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Raf1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , RAF1-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588

GTX111588 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N1C1-2] (GTX111588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588

GTX111588 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N1C1-2] (GTX111588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raf1 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX111588

GTX111588 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Raf1(GTX111588) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Raf1 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111588

GTX111588 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Raf1(GTX111588) antibody at 1:200 dilution.

REFERENCE

Application Reference

Lin CY et al. Cell Transplant 2014; IL-1β-induced mesenchymal stem cell migration involves MLCK activation via PKC signaling.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for Raf1 antibody [N1C1-2] (GTX111588). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169