Raf1 antibody [N3C3] detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Raf1 antibody [N3C3] (GTX107763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Raf1(GTX107763) antibody at 1:200 dilution.

Raf1 antibody [N3C3] detects Raf1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Raf1 stained by Raf1 antibody [N3C3] (GTX107763) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min