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USP8 antibody

Anti-USP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103747
Anti-USP8 antibody used in Immunoprecipitation (IP). GTX103747
Anti-USP8 antibody used in Western Blot (WB). GTX103747
Anti-USP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX103747

Cat. No. GTX103747

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all USP8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171647

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 8 , HumORF8 , PITA4 , SPG59 , UBPY

Database

Research Area

DATA IMAGES

Anti-USP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103747

GTX103747 ICC/IF Image

USP8 antibody detects USP8 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP8 protein stained by USP8 antibody (GTX103747) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-USP8 antibody used in Immunoprecipitation (IP). GTX103747

GTX103747 IP Image

Immunoprecipitation of USP8 protein from HCT-116 whole cell extracts using 5 μg of USP8 antibody (GTX103747).
Western blot analysis was performed using USP8 antibody (GTX103747) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-USP8 antibody used in Western Blot (WB). GTX103747

GTX103747 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP8 antibody (GTX103747) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX103747

GTX103747 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using USP8(GTX103747) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sachin Chaugule et al. Int J Mol Sci 2021; 22 (19) Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
Application : WB, IHC-P
Reactivity : Mouse
Adoro S et al. Nat Immunol 2017; 18 (7) : 780-790 Post-translational control of T cell development by the ESCRT protein CHMP5.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169