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Von Hippel Lindau antibody

Overexpression
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

Cat. No. GTX101087

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Von Hippel Lindau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Myc-DDK-tagged VHL-transfected 293T

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952549

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

von Hippel-Lindau tumor suppressor , HRCA1 , RCA1 , VHL1 , pVHL

Cellular Localization

Isoform 1: Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Isoform 3: Cytoplasm

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087

GTX101087 ICC/IF Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tang Y et al. Mol Brain 2023; 16 (1) : 74 MLKL regulates Cx43 ubiquitinational degradation and mediates neuronal necroptosis in ipsilateral thalamus after focal cortical infarction.
Yang KS et al. Sci Rep 2023; 13 (1) : 10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
Chittiboina P et al. Clin Cancer Res 2023; 29 (12) : 2199-2209 Proteostasis Modulation in Germline Missense von Hippel Lindau Disease.
Li G et al. Exp Mol Med 2022; 54 (7) : 988-998 Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9.
Tianli Chen et al. Mol Ther 2021; 29 (6) : 2134-2150 WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.
Reactivity : Human
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REVIEW

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Application Reference
Tang Y et al. Mol Brain 2023; 16 (1):74 MLKL regulates Cx43 ubiquitinational degradation and mediates neuronal necroptosis in ipsilateral thalamus after focal cortical infarction.
Yang KS et al. Sci Rep 2023; 13 (1):10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
Chittiboina P et al. Clin Cancer Res 2023; 29 (12):2199-2209 Proteostasis Modulation in Germline Missense von Hippel Lindau Disease.
Li G et al. Exp Mol Med 2022; 54 (7):988-998 Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9.
Tianli Chen et al. Mol Ther 2021; 29 (6):2134-2150 WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1):22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB, IHC-P
Reactivity : Human
Li G et al. Nat Commun 2021; 12 (1):3363 A small molecule HIF-1α stabilizer that accelerates diabetic wound healing.
Application : WB
Reactivity : Human
Xu S et al. Cell Death Dis 2019; 10 (3):191 CXCR7 promotes melanoma tumorigenesis via Src kinase signaling.
Application : WB
Reactivity : Human, Mouse
Lee SB et al. Nature 2016; 529 (7585):172-7 An ID2-dependent mechanism for VHL inactivation in cancer.
Application : WB
Reactivity : Human
Dukel M et al. J Biol Chem 2016; The Breast Cancer Tumor Suppressor TRIM29 is Expressed via ATM-Dependent Signaling in Response to Hypoxia.
Application : WB
Reactivity : Human
Li CH et al. Arch Toxicol 2014; 88 (3):659-71 Minocycline accelerates hypoxia-inducible factor-1 alpha degradation and inhibits hypoxia-induced neovasculogenesis through prolyl hydroxylase, von Hippel-Lindau-dependent pathway.
Application : WB
Reactivity : Human
Yao P et al. PLoS Biol 2013; 11 (8):e1001635 The HILDA complex coordinates a conditional switch in the 3'-untranslated region of the VEGFA mRNA.
Application : WB
Reactivity : Human
Lin CH et al. Br J Pharmacol 2013; 168 (4):920-31 Silibinin inhibits VEGF secretion and age-related macular degeneration in a hypoxia-dependent manner through the PI-3 kinase/Akt/mTOR pathway.
Application : IP
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169