Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Von Hippel Lindau antibody detects Von Hippel Lindau protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min