Vinculin antibody [N1N3]

Name: Vinculin antibody [N1N3]
Brand: GeneTex
SKU: GTX113294
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294

Anti-Vinculin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113294

Cat. No. GTX113294

Reference 10

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Vinculin products

View product citations for antibody GTX113294 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732545

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Gene ID: 7414 VCL
UniProt: P18206 VCL

Research Area

DATA IMAGES

GTX113294 WB Image

Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113294 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113294 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113294 IHC-P Image

Vinculin antibody [N1N3] detects Vinculin protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113294 ICC/IF Image

Vinculin antibody [N1N3] detects Vinculin protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Vinculin stained by Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX113294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Vinculin(GTX113294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.