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AKT antibody [N3C2], Internal

KO/KD Orthogonal Overexpression IP/MS
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

Cat. No. GTX121937

Reference 97
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Pig, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)
See all AKT products
PRODUCT

Summary

AKT antibody recognizes AKT, a serine/threonine-specific protein kinase with a predicted molecular weight of ~57 kDa. AKT is a family of three highly homologous isoforms that have more than a hundred substrates, with most of these regulated by all three proteins. Responding to many extracellular stimuli, AKT is active through major signaling nodes that include GSK3, FOXO, and TSC2/mTORC1.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

JC , BCL-1 , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , AKT1 , HA-tagged AKT2 and DDDDK-tagged AKT3-transfected 293T

Product Note

IP/MS validation was supported by references (PMID:30377414)

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723121

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT antibody , MGC99656 antibody , PKB antibody , PKB-ALPHA antibody , PRKBA antibody , RAC antibody , RAC-ALPHA antibody , AKT1 antibody , protein kinase B alpha antibody , rac protein kinase alpha antibody , RAC-alpha serine/threonine-protein kinase antibody , proto-oncogene c-Akt antibody , RAC-PK-alpha antibody , PKB alpha antibody , v-akt murine thymoma viral oncogene homolog 1 antibody , PKBB antibody , PKBBETA antibody , PRKBB antibody , RAC-BETA antibody , AKT2 antibody , protein kinase B beta antibody , murine thymoma viral (v-akt) homolog-2 antibody , protein kinase Akt-2 antibody , RAC-PK-beta antibody , PKB beta antibody , rac protein kinase beta antibody , RAC-beta serine/threonine-protein kinase antibody , v-akt murine thymoma viral oncogene homolog 2 antibody , DKFZp434N0250 antibody , PKB-GAMMA antibody , PKBG antibody , PRKBG antibody , RAC-PK-gamma antibody , RAC-gamma antibody , STK-2 antibody , AKT3 antibody , PKB gamma antibody , RAC-gamma serine/threonine-protein kinase antibody , RAC-gamma serine/threonine protein kinase antibody , v-akt murine thymoma viral oncogene homolog 3 (protein kinase B , gamma) antibody

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Research Area

DATA IMAGES

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX121937 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121937

GTX121937 ICC/IF Image

AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX121937

GTX121937 IP Image

Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937).
Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX121937

GTX121937 IHC-P Image

AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017.PMID: 28591208

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the 2022 in Nat Commun. PMID: 35169117

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the 2022 in Front Genet. PMID: 35401669

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Biomed Res Int in 2015. PMID: 26557697

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Cell Death Dis in 2014. PMID: 24457964

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Theranostics in 2019. PMID: 31754399

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Oncogene in 2018. PMID: 29993037

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

The data was published in the journal Nutrients in 2018.PMID: 30200495

REFERENCE

Application Reference

Sun Q et al. Cancer Biol Ther 2024; 25 (1) : 2321770 RND1 inhibits epithelial-mesenchymal transition and temozolomide resistance of glioblastoma via AKT/GSK3-β pathway.
Sulistyowati E et al. Int J Mol Sci 2023; 24 (21) Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress.
P Wang et al. RSC Adv 2023; Enhancing the inhibition of cell proliferation and induction of apoptosis in H22 hepatoma cells through biotransformation of notoginsenoside R1 by Lactiplantibacillus plantarum S165 into 20(S/R)-notoginsenoside R2
Application : WB
HX Du et al. Oncol Lett 2023; Eukaryotic translation initiation factor 2α kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization
W Liang et al. Probiotics Antimicrob Proteins 2023; Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota
Application : WB
Show More +
Submit a Reference

REVIEW

AKT antibody [N3C2], Internal Cat. No. GTX121937

Rating
( Average 4.3 based on 8 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 8 users reviews)
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 05-Feb-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : RWPE-1 cells with/without treatment
Amount used : 20μg
Blocking : Blocking solution, 4°C, 0.1Hr
Primary Antibody : 1:3000, 4°C, 17Hr
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 16-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW1353 cells
Amount used : 10 μg
Blocking : Block Ace (DS Parma Biomedical Co. Ltd., Osaka, Japan), 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 07-Aug-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16F10 cells
Amount used : 10 μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 12Hr
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 04-Oct-2019
Score :
Application Tested : WB
Sample Species : Japanese Medaka
Sample : HEK293T cells transfected with pCMV-medaka AKT1
Amount used : 5 μg
Blocking : 5% skim milk in TPBS, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Lane Description : Lane 1: HEK293T transfected with mock vector; Lane 2: HEK293T transfected with pCMV-medaka AKT1
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 10-Apr-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 1.66 ug
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Raw264.7 transfected pcDNA3.1(+) whole cell lysate, Lane 2: Raw264.7 transfected pCA-AKT whole cell lysate
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 13-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 20 μg
Blocking : 1% BSA in TBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 10°C, 12Hr
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Date : Anonymous submitted on 26-Mar-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : melanoma cells
Amount used : 40μg
Blocking : 5 % MTBST ( 2.5 g non-fat milk + 50 mL 1x TBST), 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Sun Q et al. Cancer Biol Ther 2024; 25 (1):2321770 RND1 inhibits epithelial-mesenchymal transition and temozolomide resistance of glioblastoma via AKT/GSK3-β pathway.
Sulistyowati E et al. Int J Mol Sci 2023; 24 (21) Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress.
P Wang et al. RSC Adv 2023; Enhancing the inhibition of cell proliferation and induction of apoptosis in H22 hepatoma cells through biotransformation of notoginsenoside R1 by Lactiplantibacillus plantarum S165 into 20(S/R)-notoginsenoside R2
Application : WB
HX Du et al. Oncol Lett 2023; Eukaryotic translation initiation factor 2α kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization
P?rez-Mora S et al. Int J Mol Sci 2023; 24 (15) BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway.
W Liang et al. Probiotics Antimicrob Proteins 2023; Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota
Application : WB
Huang C et al. Pharmaceuticals (Basel) 2023; 16 (5) α-Viniferin-Induced Apoptosis through Downregulation of SIRT1 in Non-Small Cell Lung Cancer Cells.
Russo M et al. Cancers (Basel) 2023; 15 (9) Senolytic Flavonoids Enhance Type-I and Type-II Cell Death in Human Radioresistant Colon Cancer Cells through AMPK/MAPK Pathway.
Yang NC et al. Int J Mol Sci 2023; 24 (7) The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans.
Application : WB
Reactivity : Human
Li Y et al. J Orthop Surg Res 2023; 18 (1):199 P-15 promotes chondrocyte proliferation in osteoarthritis by regulating SFPQ to target the Akt-RUNX2 axis.
Application : WB
Reactivity : Human
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Binh DHN et al. Am J Cancer Res 2023; 13 (11):5151-5173 StAR-related lipid transfer domain protein 3 (STARD3) regulates HER2 and promotes HER2-positive breast cancer progression through interaction with HSP90 and SRC signaling.
Iwashita J et al. Front Pharmacol 2022; 13:851374 Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.
Hung SY et al. Front Cell Neurosci 2022; 16:880267 Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signaling.
Jyun-Yi Du et al. J Immunol Res . 2022; 2022:8953235 Pentraxin 3 Facilitates Shrimp-Allergic Responses in IgE-Activated Mast Cells
Application : WB
Reactivity : Rat
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1):71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Yu-Hsuan Hung et al. Int J Mol Sci 2022; 23 (5) Semaphorin 6C Suppresses Proliferation of Pancreatic Cancer Cells via Inhibition of the AKT/GSK3/β-Catenin/Cyclin D1 Pathway.
Application : WB
Reactivity : Human, Mouse
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Yuhei Hasuike et al. Front Genet 2022; 13:865811 Expanded CUG Repeat RNA Induces Premature Senescence in Myotonic Dystrophy Model Cells.
Application : WB
Reactivity : Human
Yu-Xuan Ma et al. Bioact Mater 2022; 9:475-490 Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration.
Application : WB
Reactivity : Rat
Chiang HY et al. Nat Commun 2022; 13 (1):874 IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.
Application : WB
Reactivity : Mouse
Hsiao YW et al. Clin Transl Med 2022; 12 (1):e724 Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers.
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci 2022; 23 (3) Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Chi-Cheng Lu et al. Exp Ther Med 2021; 22 (2):822 Dracorhodin perchlorate enhances wound healing via β-catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes.
Application : WB
Reactivity : Human
Liqin An et al. Genes Dis 2021; 8 (4):531-544 Bone morphogenetic protein 4 (BMP4) promotes hepatic glycogen accumulation and reduces glucose level in hepatocytes through mTORC2 signaling pathway.
Application : WB, IHC-P
Reactivity : Mouse
Zhang YQ et al. Nat Commun 2021; 12 (1):6444 Dopamine D2 receptor regulates cortical synaptic pruning in rodents.
Application : WB
Reactivity : Rat
Lee KT et al. Aging (Albany NY) 2021; 13 (18):22490-22501 Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.
Application : WB
Reactivity : Human
Lo WC et al. Cell Transplant 2021; 30:9636897211045319 Platelet-Derived Biomaterials Inhibit Nicotine-Induced Intervertebral Disc Degeneration Through Regulating IGF-1/AKT/IRS-1 Signaling Axis.
Application : WB
Reactivity : Human
Wang YW et al. Transl Oncol 2021; 14 (10):101175 The significance of SMARCB1 in the pathogenesis of renal cell carcinoma with rhabdoid features.
Application : WB
Reactivity : Human
Pan CH et al. Evid Based Complement Alternat Med 2021; 2021:8466543 Preventive Effect and Mechanism of Crossostephium chinense Extract on Balloon Angioplasty-Induced Neointimal Hyperplasia.
Application : WB
Chi JY et al. Cell Death Discov 2021; 7 (1):94 Fibroblast CEBPD/SDF4 axis in response to chemotherapy-induced angiogenesis through CXCR4.
Application : WB
Reactivity : Human
Terasaki M et al. Nutr Cancer 2021;:1-16 A Marine Carotenoid of Fucoxanthinol Accelerates the Growth of Human Pancreatic Cancer PANC-1 Cells.
Terasaki M et al. Cancer Genomics Proteomics 2021; 18 (2):133-146 A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell.
Application : WB
Reactivity : Mouse
Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.
Application : WB
Reactivity : Human
Chiang AJ et al. Biomolecules 2020; 11 (1) UBE2C Drives Human Cervical Cancer Progression and Is Positively Modulated by mTOR.
Application : WB
Reactivity : Human
Lee CH et al. Cancers (Basel) 2020; 12 (11) LDOC1 Suppresses Microbe-Induced Production of IL-1β in Human Normal and Cancerous Oral Cells through the PI3K/Akt/GSK-3β Axis.
Application : WB
Reactivity : Human
Pan CH et al. Chem Biol Interact 2020; 332:109304
Application : WB
Reactivity : Human
Lo WC et al. Cell Transplant 2020; 29:9.6369E+14 Amelioration of Nicotine-Induced Osteoarthritis by Platelet-Derived Biomaterials Through Modulating IGF-1/AKT/IRS-1 Signaling Axis.
Application : WB
Reactivity : Human
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Li J et al. J Ethnopharmacol 2020; 257:112796 Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signaling.
Application : WB, IHC-P
Reactivity : Rat
Chen SY et al. Stem Cells Transl Med 2020; 9 (4):499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Xie MY et al. Biochem Pharmacol 2020; 175:113898 Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage.
Application : WB
Reactivity : Pig
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Li H et al. Neurotox Res 2020; 37 (4):996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.
Application : WB
Reactivity : Rat
Wang J et al. Am J Physiol Cell Physiol 2020; (Epub) The Physiological Modulation of Intracellular kinases on Hippocampal Gamma Oscillation in Vitro.
Application : WB
Reactivity : Rat
Nguyen HD et al. Cancers (Basel) 2019; 11 (12) The α9 Nicotinic Acetylcholine Receptor Mediates Nicotine-Induced PD-L1 Expression and Regulates Melanoma Cell Proliferation and Migration.
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Chen H et al. Theranostics 2019; 9 (26):8321-8331 Pten loss in Lgr5+ hair follicle stem cells promotes SCC development.
Application : WB
Reactivity : Mouse
Bera R et al. J Hematol Oncol 2019; 12 (1):104 RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.
Application : WB
Reactivity : Human
Li X et al. ACS Nano 2019; Gadofullerene Nanoparticles Reverse Dysfunctions of Pancreas and Improve Hepatic Insulin Resistance for Type 2 Diabetes Mellitus Treatment.
Application : WB
Reactivity : Human
Ko PH et al. Sci Rep 2019; 9 (1):2147 Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Application : WB
Reactivity : Human
Huang C et al. J Agric Food Chem 2019; 67 (10):2818-2830 Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
Application : WB
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.
Lai YJ et al. J Cell Physiol 2018; (Epub) CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chen ST et al. Oncogene 2018; (Epub) Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.
Application : WB
Reactivity : Human
Liao WC et al. PLoS One 2018; 13 (6):e0198364 DSE promotes aggressive glioma cell phenotypes by enhancing HB-EGF/ErbB signaling.
Application : WB
Reactivity : Human
Wu PY et al. Int J Mol Sci 2018; 19 (4) Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
Application : WB
Reactivity : Mouse
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Liu J et al. Oncol Lett 2017; 14 (5):5711-5718 miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Chen X et al. Sci Rep 2017; 7 (1):13248 hydrogen sulphide treatment increases * sensitivity and improves oxidant metabolism through the camkkbeta-ampk pathway in pa-induced ir c2c12 cells.
Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Chen H et al. PLoS One 2017; 12 (6):e0178969 AKT and its related molecular feature in aged mice skin.
Application : WB
Reactivity : Mouse
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Application : WB
Reactivity : Human
Viedma-Rodriguez R et al. Mol Cell Biochem 2017; (Epub) epsilon-aminocaproic acid prevents high glucose and * induced-invasiveness in mda-mb-231 breast cancer cells, modulating the plasminogen activator system.
Application : WB
Reactivity : Human
Liu J et al. Biotechnol Lett 2017; (Epub) MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Application : WB
Reactivity : Human
Chen X et al. Redox Biol 2017; 12:987-1003 The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Application : WB
Reactivity : Rat
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Chao W et al. Molecules 2017; 22 (4) 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Application : WB
Reactivity : Human
Wang X et al. Nat Commun 2017; 8:14091 Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF.
Application : WB, IHC-Fr
Reactivity : Mouse
Wang HH et al. Tumour Biol 2017; 39 (4):1.01E+15 targeting *-like growth factor-binding protein-3 by microrna-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer.
Reactivity : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Kuo YH et al. BMC Complement Altern Med 2017; 17 (1):71 N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Application : WB
Reactivity : Mouse
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Hsieh CH et al. Drug Des Devel Ther 2016; 10:2947-2957 A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Application : WB
Reactivity : Mouse
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Lin CS et al. Int J Mol Sci 2016; 17 (6) Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Application : WB
Reactivity : Human
Jan CI et al. Int J Biol Sci? 2016; 12 (7):786-798 Fenofibrate Suppresses Oral Tumorigenesis via Reprogramming Metabolic Processes: Potential Drug Repurposing for Oral Cancer.
Application : WB
Reactivity : Human
Hung CC et al. Oncotarget 2015; 6 (6):4110-25 p22phox confers resistance to *, by blocking its entry into the nucleus.
Application : WB
Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Carpentieri A et al. Cell Death Dis 2015; 6:e1974 Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor.
Masola V et al. BMC Gastroenterol 2015; 15:118 epithelial to mesenchymal transition in the liver field: the double face of * in vitro.
Gabriela Vallejo-Flores et al. BioMed Research International 2015; Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation
Application : WB, IP
Reactivity : Human
Huang RF et al. Int J Nanomedicine 2015; 10:2823-46 Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.
Application : WB
Reactivity : Human
Huang YL et al. Hum Mol Genet 2015; 24:3506-17 FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Application : WB
Reactivity : Human
Sun W et al. Cell Death Dis 2014; 5:e1020 Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
Application : WB
Reactivity : Human
Ho WL et al. Oncotarget 2014; 5 (23):12247-59 GALNT2 suppresses malignant phenotypes through IGF-1 receptor and predicts favorable prognosis in neuroblastoma.
Application : WB
Reactivity : Human
Liao WY et al. J Pathol 2014; Heparin Cofactor II Enhances Cell Motility and Promotes Metastasis in Non-Small-Cell Lung Cancer.
Application : WB
Reactivity : Human
Kim JW et al. Genes Cancer 2014; 5 (43226):165-74 hoxa10 is associated with * resistance through regulation of the homologous recombinant dna repair pathway in glioblastoma cell lines.
Application : WB
Reactivity : Human
Wu YM et al. Cancer Res 2013; 73 (17):5580-90 C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization.
Application : WB
Reactivity : Human
SDS
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Proclin.pdf
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