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AKT1 antibody

Overexpression IP/MS
Anti-AKT1 antibody used in Immunoprecipitation (IP). GTX110613
Anti-AKT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110613
Anti-AKT1 antibody used in Western Blot (WB). GTX110613
Anti-AKT1 antibody used in Western Blot (WB). GTX110613
Anti-AKT1 antibody used in Western Blot (WB). GTX110613

Cat. No. GTX110613

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa cell transfect with AKT , Mouse liver

Product Note

IP/MS validation was supported by references (PMID:30377404)

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617128

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT1 antibody used in Immunoprecipitation (IP). GTX110613

GTX110613 IP Image

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments.
IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells.
A. 30 μg whole cell extract of Akt1-protein expressing 293T cell
B. Control with 3 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613)
10 % SDS-PAGE
The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-AKT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110613

GTX110613 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1(GTX110613) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKT1 antibody used in Western Blot (WB). GTX110613

GTX110613 WB Image

Overexpression

Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX110613 diluted at 1:10000

Anti-AKT1 antibody used in Western Blot (WB). GTX110613

GTX110613 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody (GTX110613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody used in Western Blot (WB). GTX110613

GTX110613 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX110613 diluted at 1:1000

REFERENCE

Application Reference

Vadhan A et al. Biomedicines 2022; 10 (10) CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2.
Carmen Cervellera et al. Molecules 2021; 26 (19) STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line.
Application : WB
Reactivity : Human
Zhang Z et al. Oncol Rep 2019; 41 (1) : 619-629 MicroRNA-296 inhibits colorectal cancer cell growth and enhances apoptosis by targeting ARRB1-mediated AKT activation.
Application : WB
Reactivity : Mouse
Okabe S et al. Ann Hematol 2018; (Epub) Copanlisib, a novel phosphoinositide 3-kinase inhibitor, combined with carfilzomib inhibits multiple myeloma cell proliferation.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
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Submit a Reference

REVIEW

AKT1 antibody Cat. No. GTX110613

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-AKT1 antibody used in Western Blot (WB). GTX110613
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Vadhan A et al. Biomedicines 2022; 10 (10) CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2.
Carmen Cervellera et al. Molecules 2021; 26 (19) STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line.
Application : WB
Reactivity : Human
Zhang Z et al. Oncol Rep 2019; 41 (1):619-629 MicroRNA-296 inhibits colorectal cancer cell growth and enhances apoptosis by targeting ARRB1-mediated AKT activation.
Application : WB
Reactivity : Mouse
Okabe S et al. Ann Hematol 2018; (Epub) Copanlisib, a novel phosphoinositide 3-kinase inhibitor, combined with carfilzomib inhibits multiple myeloma cell proliferation.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub):142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Chen YJ et al. Gynecol Oncol 2013; 131 (3):734-43 The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169