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Galectin 3 antibody

KO/KD Overexpression
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX125897
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897
Anti-Galectin 3 antibody used in ELISA (ELISA). GTX125897
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

Cat. No. GTX125897

Reference 7
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Galectin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635156, Detection: GTX125897
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A549 , HCT116 , LGALS3 transfected 293T , rat colon , HeLa , AsPC-1

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Galectin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178427

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Nucleus

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

GTX125897 WB Image

KO/KD

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX125897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

GTX125897 WB Image

Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX125897) dilution: 1:1000

Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX125897

GTX125897 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin3 antibody(GTX125897) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

GTX125897 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysate
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX125897 diluted at 1:1000

Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

GTX125897 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX125897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in ELISA (ELISA). GTX125897

GTX125897 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635156 as capture antibody at concentration of 5 μg/mL and GTX125897 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897

GTX125897 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HCT116
12% SDS PAGE
GTX125897 diluted at 1:1000

REFERENCE

Application Reference

Sbroscia M et al. Sci Rep 2020; 10 (1) : 13342 Thyroid cancer diagnosis by Raman spectroscopy.
Application : IHC-P, IHC-Fr
Reactivity : Human
Li FY et al. Glycobiology 2018; (Epub) Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner.
Application : WB
Reactivity : Human
Lin GL et al. PLoS One 2017; 12 (10) : e0185625 modulation of the mrna-binding protein hur as a novel reversal mechanism of *-triggered multidrug resistance in colorectal cancer cells.
Application : WB
Reactivity : Human
Brandi J et al. J Proteomics 2016; 136 : 43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; 10 (12) : 3086-100 Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Galectin 3 antibody Cat. No. GTX125897

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-Galectin 3 antibody used in Western Blot (WB). GTX125897
Date : Anonymous submitted on 13-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX125897
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Liver tissue
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : control/diabetic mouse (250x lens)
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX125897
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Liver tissue
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : control/diabetic mouse (400x lens)
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169