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Rabbit Anti-Mouse kappa Light chain antibody [RM103]

Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858
Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858
Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858

Cat. No. GTX60858

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM103

Isotype

 IgG

Application

 WB, FACS, IP, ELISA

Reactivity

 Mouse
Package
100 μg ($369)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1μg/mL - 0.5μg/mL
FACS Assay dependent
IP Assay dependent
ELISA 0.005μg/mL - 0.2μg/mL
Not tested in other applications.

Product Note

This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of RM103 has been engineered to eliminate Fc receptor binding.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG

Target Isotype

IgG (kappa light chain)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Summary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 WB Image

WB analysis of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103].
Dilution : 0.2μg/ml

Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 ELISA Image

ELISA analysis of mouse immunoglobulins (50 ng/well) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103]. No cross reactivity with the lamda lightchain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ).
Dilution : 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody

Rabbit Anti-Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103].

REFERENCE

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REVIEW

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Package List Price ($)
$ 369