APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2 μg/ml |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Does not cross-react with PRMT1, 2, 4, 5, or 6.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
synthetic peptide corresponding to amino acids 501-514 of rat PRMT3 conjugated to KLH.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein arginine methyltransferase 3 , Hrmt1l3
Cellular Localization
Cytoplasm
Background
Posttranslational modifications of proteins play an important role in the regulation of protein function, stability and localization. Such modifications occur on different amino acids and include phosphorylation, glycosylation, acetylation, or methylation. Arginine methylation is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. In type I PRMTs, the methylate arginine is found in different motifs such as the Arg-Gly-Gly-rich region (RGG motif) in many RNA binding proteins or the Arg-Xaa-Arg motif in poly(A)- binding protein II. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT3 is expressed in several tissues such as heart, small intestine, kidney, ovary, testis, thyroid, and cortex with a cytoplasmic localization within the cells. PRMT3 consists of two important domains in its N-terminal region (a C2H2 zinc-finger motif and a tyrosine-phosphorylation consensus sequence), which are probably important for regulation and catalytic activity.
Database
Research Area
REFERENCE
There are currently no references for PRMT3 antibody [PRMT3-367] (GTX12190). Be the first to share your publications with this product.
REVIEW
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