POLY-L-LYSINE was diluted in 1:10 with distilled water, soak clean slide in this solution at room temperature for 5 minutes (Extend to 10 minutes in case of room temperature below 15ºC). And the container should be plastic, such as plastic section box, plastic bottle, etc. Get slide out, make it dried or baked at a place with no dust. Seal and store at 4ºC for half of a year. The glass container is not recommended to hold diluent solution to avoid affecting the result.