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Porcine circovirus type 2 / PCV2 replicase antibody

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638

Cat. No. GTX133638

Reference 2
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Porcine circovirus type 2
Package
100 μl ($399),
25 μl ($169)
See all Porcine circovirus type 2 replicase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PCV2-infected PK-15

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Porcine circovirus type 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Porcine circovirus type 2 / PCV2 replicase (PCV2 virus (strain AUT1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Porcine circovirus Replicase , Porcine circovirus Rep , PCV Replicase , PCV Rep

Research Area

DATA IMAGES

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638

GTX133638 WB Image

Non-infected (–) and infected (+) PK-15 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638

GTX133638 ICC/IF Image

Porcine circovirus type 2 / PCV2 replicase antibody detects Porcine circovirus type 2 / PCV2 replicase protein by immunofluorescent analysis.
Sample: Uninfected and infected PK-15 cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: Porcine circovirus type 2 / PCV2 replicase stained by Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638) diluted at 1:50.
Image courtesy of Dr. Karuppannan from Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

REFERENCE

Application Reference

Ouyang Y et al. Vet Res 2024; 55 (1) : 22 Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Pi?train pigs.
Zhang S et al. Molecules 2019; 24 (23) Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification.
Submit a Reference

REVIEW

Porcine circovirus type 2 / PCV2 replicase antibody Cat. No. GTX133638

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638
Date : Anonymous submitted on 24-Sep-2019
Score :
Application Tested : WB
Sample Species : PCV2
Sample : PAM cells
Amount used : 30 μg
Blocking : 5 % non-fat milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638
Date : Anonymous submitted on 22-Jun-2018
Score :
Application Tested : ICC/IF
Sample Species : PCV2
Sample : PK-15 cells
Blocking : 1% BSA in PBS with 0.05% Tween, 37°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS, pH7.4, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.2% TritonX-100 in PBS, 25°C, 40 minHr
Primary Antibody : 1:50, 37°C, 1Hr
Figure Note : PCV2 non-infected (upper left) and infected (upper right, lower left and lower right) PK-15 cells. Image courtesy of Dr. Karuppannan from the lab "Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA".
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169