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United States (US)

Pronase reagent (Ready-to-use)

Cat. No. GTX73181

Reference 1

Application

 IHC
Package
100 ml ($179)
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PRODUCT

Summary

Pronase Reagent

APPLICATION

Application Note

For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.

PROPERTIES

Form

Liquid

Storage

Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

REFERENCE

Application Reference

Sun JD et al. Clin Cancer Res 2012; 18 (3) : 758-70 Selective tumor hypoxia targeting by hypoxia-activated prodrug TH-302 inhibits tumor growth in preclinical models of cancer.
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Package List Price ($)
$ 179