SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9]

Name: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9]
Brand: GeneTex
SKU: GTX632602
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602

Cat. No. GTX632602

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	3C9
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	SARS Coronavirus, SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) ORF7a products

View product citations for antibody GTX632602 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Observed MW

15 kDa. The observed M.W. is based on the following publication. PMID: 15220404

Positive Control

ORF7a-transfected 293T cell

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV ORF7a protein (16-111 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888320

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV ORF7a , SARS-coV-2 ORF7a , COVID-19 ORF7a

Research Area

DATA IMAGES

GTX632602 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.

GTX632602 WB Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV ORF7a protein by western blot analysis. Non-transfected (-) and SARS-CoV ORF7a-transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 15220404

GTX632602 ICC/IF Image

ICC/IF analysis of Vero E6 cells expressing ORF7a using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

GTX632602 WB Image

WB analysis of SARS-CoV-infected Vero E6 cells harvested at different time points postinfection using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

GTX632602 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a stained by SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).