SOX2 antibody [GT1352]

Name: SOX2 antibody [GT1352]
Brand: GeneTex
SKU: GTX627405
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405

Anti-SOX2 antibody [GT1352] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627405

Anti-SOX2 antibody [GT1352] used in IHC (Paraffin sections) (IHC-P). GTX627405

Anti-SOX2 antibody [GT1352] used in IHC (Frozen sections) (IHC-Fr). GTX627405

Anti-SOX2 antibody [GT1352] used in Flow cytometry (FACS). GTX627405

Cat. No. GTX627405

Reference 6

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1352
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all SOX2 products

View product citations for antibody GTX627405 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human ESC , mouse ESC , NT2D1

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11165499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Gene ID: 6657 SOX2
UniProt: P48431 SOX2

Research Area

DATA IMAGES

GTX627405 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627405 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX627405 ICC/IF Image

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

GTX627405 IHC-P Image

SOX2 antibody [GT1352] detects SOX2 protein at nucleus on by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
SOX2 antibody [GT1352] (GTX627405) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX627405 WB Image

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627405 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX108711) diluted at 1:1000.
Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:250.

GTX627405 IHC-P Image

SOX2 antibody [GT1352] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX627405 WB Image

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg mouse ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627405 FACS Image

SOX2 antibody [GT1352] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1352] dilution: 1:50

REFERENCE

Application Reference

Kitagawa T et al. PLoS One 2022; 17 (10) : e0269077 AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells.

Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.

Application : WB

Reactivity : Human

Jakub Ska?bania et al. Pharmacol Rep 2021; 73 (4) : 1164-1169 Chlorpromazine affects the numbers of Sox-2, Musashi1 and DCX-expressing cells in the rat brain subventricular zone.

Application : IHC-P

Reactivity : Mouse

Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020 : 4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.

Yang SC et al. FASEB J 2019; 33 (9) : 10577-10592 Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2.

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