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AMPK alpha 2 antibody

KO/KD
Anti-AMPK alpha 2 antibody used in Immunoprecipitation (IP). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

Cat. No. GTX103487

Reference 9
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all AMPK alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

NIH-3T3 , 293T , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:29753072)

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951385

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 2 , AMPK , AMPK2 , AMPKa2 , PRKAA

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 2 antibody used in Immunoprecipitation (IP). GTX103487

GTX103487 IP Image

AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 μg of AMPK alpha 2 antibody (GTX103487) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487

GTX103487 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487

GTX103487 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX103487 diluted at 1:1000

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103487

GTX103487 ICC/IF Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm, nucleus and vesicle by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Gao J et al. Redox Biol 2019; 28 : 101345
Application : WB
Reactivity : Mouse
Wang H et al. Free Radic Biol Med 2018; 121 : 202-214 AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.
Application : WB
Reactivity : Mouse
Wu W et al. J Physiol Biochem 2017; (Epub) Muscle-specific deletion of Prkaa1 enhances skeletal muscle lipid accumulation in mice fed a high-fat diet.
Application : WB
Reactivity : Mouse
Guo Y et al. Arch Biochem Biophys 2016; 600 : 56-60 AMP-activated kinase α2 deficiency protects mice from denervation-induced skeletal muscle atrophy.
Application : WB
Reactivity : Mouse
Zhou X et al. Nutr Metab (Lond) 2015; 12 : 10 AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

AMPK alpha 2 antibody Cat. No. GTX103487

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Epidydimal white adipocytes tissue lysate
Amount used : 40 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Figure Note : NCD: Normal chow diet
HFD: High fat diet
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Date : Anonymous submitted on 31-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 40 μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Submit a Review
Application Reference
Gao J et al. Redox Biol 2019; 28:101345
Application : WB
Reactivity : Mouse
Wang H et al. Free Radic Biol Med 2018; 121:202-214 AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.
Application : WB
Reactivity : Mouse
Wu W et al. J Physiol Biochem 2017; (Epub) Muscle-specific deletion of Prkaa1 enhances skeletal muscle lipid accumulation in mice fed a high-fat diet.
Application : WB
Reactivity : Mouse
Guo Y et al. Arch Biochem Biophys 2016; 600:56-60 AMP-activated kinase α2 deficiency protects mice from denervation-induced skeletal muscle atrophy.
Application : WB
Reactivity : Mouse
Zhou X et al. Nutr Metab (Lond) 2015; 12:10 AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid.
Application : WB
Reactivity : Mouse
Lee YZ et al. Oncotarget 2014; 5 (15):6087-101 Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.
Application : WB
Reactivity : Human
Zhou X et al. J Physiol Biochem 2014; Selenium-enriched exopolysaccharides improve skeletal muscle glucose uptake of diabetic KKAy mice via AMPK pathway.
Lin YY et al. Nature 2012; 482 (7384):251-5 Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Reactivity : Human
Hsu YF et al. Biochim Biophys Acta 2012; 1820 (2):104-15 Trichostatin A and sirtinol suppressed survivin expression through AMPK and p38MAPK in HT29 colon cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169