PRODUCT
Summary
Carbonic Anhydrase IX antibody (CA9 antibody) detects carbonic anhydrase 9, a ~50 kDa transmembrane glycoprotein. CA9 expression is enhanced by HIF1-alpha signaling in various biological processes, including cell proliferation and transformation. Found in many tissues, CA9 overexpression promotes tumor growth in various cancers.
APPLICATION
Application Note
For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10μg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Product Note
Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Conjugation
Unconjugated
RRID
AB_11173476
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
carbonic anhydrase 9 , CAIX , MN
Cellular Localization
Nucleus , Cell membrane , Nucleolus
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Database
Research Area
DATA IMAGES
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GTX70020 IHC-P Image
Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70020 IHC-P Image
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
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GTX70020 FACS Image
Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.
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GTX70020 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
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GTX70020 WB Image
Sample (30 μg HeLa whole cell lysate)
A: 24 hr Untreated
B: 24 hr treatment with 100μM CoCl2
C: 24 hr treatment with 200μM CoCl2
D: 48 hr Untreated
E: 48 hr treatment with 100μM CoCl2
F: 48 hr treatment with 200μM CoCl2
Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70020 IHC-P Image
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
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GTX70020 IHC-P Image
Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
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GTX70020 WB Image
The data was published in the journal Oncol Lett in 2015. PMID: 26622817
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GTX70020 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25420025
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GTX70020 IHC-P Image
The data was published in the journal PLoS One in 2015. PMID: 25738958
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GTX70020 WB Image
The data was published in the journal Oncol Lett in 2015. PMID: 26622817
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REFERENCE
REVIEW
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