APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 1A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240645
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coronin 1A , CLABP , CLIPINA , HCORO1 , IMD8 , TACO , p57
Cellular Localization
Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , phagosome membrane
Background
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106424 IHC-P Image
Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106424 IHC-P Image
Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106424 IHC-P Image
Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106424 IHC-P Image
Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106424 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Coronin 1A(GTX106424) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106424 IHC-Fr Image
Coronin 1A antibody [C3], C-term detects Coronin 1A Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX106424 ICC/IF Image
Coronin 1A antibody [C3], C-term detects Coronin 1A protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX106424 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:1000.
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REFERENCE
There are currently no references for Coronin 1A antibody [C3], C-term (GTX106424). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Coronin 1A antibody [C3], C-term (GTX106424). Be the first to share your experience with this product.