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Coronin 1A antibody [C3], C-term

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106424
Anti-Coronin 1A antibody [C3], C-term used in Western Blot (WB). GTX106424

Cat. No. GTX106424

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coronin 1A , CLABP , CLIPINA , HCORO1 , IMD8 , TACO , p57

Cellular Localization

Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , phagosome membrane

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coronin 1A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106424

GTX106424 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Coronin 1A(GTX106424) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Coronin 1A antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX106424

GTX106424 IHC-Fr Image

Coronin 1A antibody [C3], C-term detects Coronin 1A Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-Coronin 1A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106424

GTX106424 ICC/IF Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Coronin 1A antibody [C3], C-term used in Western Blot (WB). GTX106424

GTX106424 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:1000.

REFERENCE

There are currently no references for Coronin 1A antibody [C3], C-term (GTX106424). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Coronin 1A antibody [C3], C-term (GTX106424). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169