APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 4 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)
Product Note
At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2.The immunogen is located within the last 50 amino acids of ORAI2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ORAI calcium release-activated calcium modulator 2 , C7orf19 , CBCIP2 , MEM142B , TMEM142B
Cellular Localization
Membrane
Background
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
Database
Research Area
DATA IMAGES
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GTX85443 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX85443 ORAI2 antibody.
Working concentration : 20 μg/ml
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GTX85443 WB Image
WB analysis of Jurkat cell lysate using GTX85443 ORAI2 antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml
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GTX85443 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX85443 ORAI2 antibody.
Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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