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ARID5B antibody

Orthogonal
Anti-ARID5B antibody used in IHC (Paraffin sections) (IHC-P). GTX131249
Anti-ARID5B antibody used in Western Blot (WB). GTX131249
Anti-ARID5B antibody used in IHC (Paraffin sections) (IHC-P). GTX131249
Anti-ARID5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131249

Cat. No. GTX131249

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARID5B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

HeLa nuclear extract

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARID5B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886449

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 5B , DESRT , MRF-2 , MRF2

Cellular Localization

Nucleus

Background

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-ARID5B antibody used in IHC (Paraffin sections) (IHC-P). GTX131249

GTX131249 IHC-P Image

ARID5B antibody detects ARID5B protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ARID5B stained by ARID5B antibody (GTX131249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID5B antibody used in Western Blot (WB). GTX131249

GTX131249 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID5B antibody (GTX131249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID5B antibody used in IHC (Paraffin sections) (IHC-P). GTX131249

GTX131249 IHC-P Image

ARID5B antibody detects ARID5B protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ARID5B stained by ARID5B antibody (GTX131249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131249

GTX131249 ICC/IF Image

ARID5B antibody detects ARID5B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID5B stained by ARID5B antibody (GTX131249) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169