Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Luciferase [Photobacterium fischerii].
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl, 1% BSA, 50% Glycerol
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
10 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Luciferase from Photobacterium fischerii
Purification
IgG fraction
IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against 0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2.
Conjugation
Biotin
Ratio : 10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per Rabbit IgG molecule.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.