APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human CCR4 Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human CCR4. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine receptor 4 , CC-CKR-4 , CD194 , CKR4 , CMKBR4 , ChemR13 , HGCN:14099 , K5-5
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX638830 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638830 WB Image
Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638830 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638830 WB Image
Unboiled SW480 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)
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GTX638830 IHC-P Image
CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.CCR4 stained by CCR4 antibody [HL2481] (GTX638830) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638830 IHC-P Image
CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat thymus gland.CCR4 stained by CCR4 antibody [HL2481] (GTX638830) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638830 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CCR4 antibody [HL2481] (GTX638830). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CCR4 antibody [HL2481] (GTX638830). Be the first to share your experience with this product.