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CD3 antibody [17A2] (PE)

Anti-CD3 antibody [17A2] (PE) used in Flow cytometry (FACS). GTX01458-08

Cat. No. GTX01458-08

Host

Rat

Clonality

Monoclonal

Clone Name

17A2

Isotype

IgG2b

Application

FACS

Reactivity

Mouse
Package
100 μg ($189)
PRODUCT

Summary

The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3 antigen, delta polypeptide , CD3 antigen, epsilon polypeptide , CD3 antigen, gamma polypeptide , CD247 antigen , T3d , AI504783 , CD3 , CD3epsilon , T3e , Ctg-3 , Ctg3 , T3g , 4930549J05Rik , A430104F18Rik , AW552088 , Cd3 , Cd3-eta , Cd3-zeta , Cd3h , Cd3z , Cd3zeta , T3z , Tcrk , Tcrz

Cellular Localization

Cell membrane , Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD3 antibody [17A2] (PE) used in Flow cytometry (FACS). GTX01458-08

GTX01458-08 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01458-08 CD3 antibody [17A2] (PE).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.5 μg (5 μl)

REFERENCE

REVIEW

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Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 189