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CD34 antibody [HL1895]

Anti-CD34 antibody [HL1895] used in IHC (Paraffin sections) (IHC-P). GTX637644
Anti-CD34 antibody [HL1895] used in Flow cytometry (FACS). GTX637644

Cat. No. GTX637644

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1895

Isotype

IgG

Application

IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody was raised against human CD34 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CD34. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD34 molecule

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [HL1895] used in IHC (Paraffin sections) (IHC-P). GTX637644

GTX637644 IHC-P Image

CD34 antibody [HL1895] detects CD34 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CD34 stained by CD34 antibody [HL1895] (GTX637644) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD34 antibody [HL1895] used in Flow cytometry (FACS). GTX637644

GTX637644 FACS Image

CD34 antibody [HL1895] (GTX637644) detects CD34 protein by flow cytometry analysis.
Sample: HUVEC and Jurkat cell.
Gray: Rabbit IgG Isotype control.
Red: CD34 antibody [HL1895] (GTX637644) dilution: 1:200.
Acquisition of 30,000 events were collected for FACS analysis.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations