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CXCR2 antibody [HL2604]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056
Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in IHC (Paraffin sections) (IHC-P). GTX639056
Anti-CXCR2 antibody [HL2604] used in Functional Assay (Functional Assay). GTX639056

Cat. No. GTX639056

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2604

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Functional Assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CXCR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Observed MW

40-95 kDa.

Product Note

This antibody was raised against human CXCR2 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CXCR2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 2 , CD182 , CDw128b , CMKAR2 , IL8R2 , IL8RA , IL8RB

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Comparison Orthogonal

Unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and competitor's antibody (Clone 48311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Comparison Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056

GTX639056 ICC/IF Image

Comparison

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:200 and competitor's antibody (Highly Cited Competitor) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056

GTX639056 ICC/IF Image

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in MeOH.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Overexpression

Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Orthogonal

Unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCR2 antibody [HL2604] used in IHC (Paraffin sections) (IHC-P). GTX639056

GTX639056 IHC-P Image

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR2 antibody [HL2604] used in Functional Assay (Functional Assay). GTX639056

GTX639056 Functional Assay Image

CXCR2 antibody [HL2604] (GTX639056) specifically detects CXCR2 protein on a VirDTM-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:1000 dilution.

Yellow: CXCR2
Blue: CXCR1, CXCR3, CXCR4, CXCR5, or CXCR6
White: Positive control

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations