APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse heart , A431 , Molt-4 , rat heart
Predict Reactivity
Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952325
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112770 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000.
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GTX112770 WB Image
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: Molt-4 (GTX27912) 12% SDS PAGE GTX112770 diluted at 1:1000
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GTX112770 WB Image
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE GTX112770 diluted at 1:10000
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REFERENCE
There are currently no references for Cardiac Troponin I antibody [N1C3-2] (GTX112770). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cardiac Troponin I antibody [N1C3-2] (GTX112770). Be the first to share your experience with this product.