GeneTex

  • Country / Location Selection

United States (US)

ERK5 antibody [GT1258]

Recombinant antibody
Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855
Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855
Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855
Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855
Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855
Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855
Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855

Cat. No. GTX02855

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1258

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all ERK5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Observed MW

120 kDa. The observed M.W. is based on the following publication. PMID: 15692064

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ERK5

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 7 , BMK1 , ERK4 , ERK5 , PRKM7

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855

GTX02855 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERK5 antibody [GT1258] (GTX02855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15692064

Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855

GTX02855 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERK5 antibody [GT1258] (GTX02855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15692064

Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855

GTX02855 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100

Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855

GTX02855 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100

Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855

GTX02855 WB Image

WB analysis of various samples using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:500
Loading : 25μg

Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855

GTX02855 IHC-P Image

IHC-P analysis of human colon tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100

Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855

GTX02855 WB Image

WB analysis of various samples using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:500
Loading : 25μg

REFERENCE

There are currently no references for ERK5 antibody [GT1258] (GTX02855). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ERK5 antibody [GT1258] (GTX02855). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 419