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GFP antibody [GT7312]

Anti-GFP antibody [GT7312] used in Immunoprecipitation (IP). GTX628894
Anti-GFP antibody [GT7312] used in ELISA (ELISA). GTX628894
Anti-GFP antibody [GT7312] used in Western Blot (WB). GTX628894

Cat. No. GTX628894

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT7312

Isotype

 IgG2a

Application

 WB, IP, ELISA, Sandwich ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX628894 , Detection: GTX113617
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2773724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-GFP antibody [GT7312] used in Immunoprecipitation (IP). GTX628894

GTX628894 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628894) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628894) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT7312] used in ELISA (ELISA). GTX628894

GTX628894 ELISA Image

ELISA detection of GFP using GTX628894 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628894, 2μg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT7312] used in Western Blot (WB). GTX628894

GTX628894 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody [GT7312] (GTX628894) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sreeja JS et al. Cell Cycle 2023; 22 (3) : 361-378 The centrosomal recruitment of γ-tubulin and its microtubule nucleation activity is α-fodrin guided.
Sreeja JS et al. FEBS Lett 2019; 593 (11) : 1154-1165 Binding of alpha-fodrin to gamma-tubulin accounts for its role in the inhibition of microtubule nucleation.
Boubriak II et al. PLoS One 2017; 12 (5) : e0177990 Stress-induced release of Oct-1 from the nuclear envelope is mediated by JNK phosphorylation of lamin B1.
Application : IP
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations