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GFP antibody

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

Cat. No. GTX13970

Reference 38

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC, IHC (Free Floating)

Reactivity

 Species independent
Package
100 μl ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA Assay dependent
IHC 1:2000-1:5000
IHC (Free Floating) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Sodium Phosphate (10 mM, pH7.2) buffered isotonic saline (0.9%), 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant.

Purification

Purified by antigen-affinity chromatography
Serile filtered.

Conjugation

Unconjugated

RRID

AB_371416

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

IHC analysis of paraformaldehyde-fixed (4%) neonatal mouse brain using GTX13970 GFP antibody.

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970

GTX13970 IHC-Wm Image

The data was published in the journal Elife in 2017.PMID: 28832322

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Sci Rep in 2017. PMID: 28959031

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Elife in 2014. PMID: 25490154

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

REFERENCE

Application Reference

Williamson MR et al. Nat Commun 2023; 14 (1) : 6341 Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.
X Meng et al. Nature 2023; Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment
Application : ICC/IF
Uttley K et al. Life Sci Alliance 2023; 6 (11) Unique activities of two overlapping PAX6 retinal enhancers.
Jin C et al. Bioeng Transl Med 2023; 8 (2) : e10448 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Abdel-Razek O et al. Front Mol Biosci 2023; 10 : 1292076 Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo.
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REVIEW

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Application Reference
Williamson MR et al. Nat Commun 2023; 14 (1):6341 Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.
X Meng et al. Nature 2023; Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment
Application : ICC/IF
Uttley K et al. Life Sci Alliance 2023; 6 (11) Unique activities of two overlapping PAX6 retinal enhancers.
Jin C et al. Bioeng Transl Med 2023; 8 (2):e10448 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Abdel-Razek O et al. Front Mol Biosci 2023; 10:1292076 Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo.
Trolese MC et al. Mol Ther 2022; 30 (8):2760-2784 Boosting the peripheral immune response in the skeletal muscles improved motor function in ALS transgenic mice.
Kim S et al. Neuron 2022; 110 (8):1371-1384.e7 Co-packaging of opposing neurotransmitters in individual synaptic vesicles in the central nervous system.
Application : IHC
Revah O et al. Nature 2022; 610 (7931):319-326 Maturation and circuit integration of transplanted human cortical organoids.
Domenico M Guarascio et al. J Physiol 2021; 599 (15):3697-3714 Functional expression of TMEM16A in taste bud cells.
Application : IHC-Fr
Peu Santra et al. Dis Model Mech 2021; 14 (7) Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts.
Application : IHC
Takahashi N et al. Curr Biol 2021; 31 (18):4148-4155.e4 Thalamic input to motor cortex facilitates goal-directed action initiation.
Kim S. et al. bioRxiv 2021; (Epub) Biophysical demonstration of co-packaging of glutamate and GABA in individual synaptic vesicles in the central nervous system.
DeMarco E et al. Front Neuroanat 2021; 15:636683 Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis.
Application : IHC-Fr
Yuki Miura et al. Nat Biotechnol 2020; 12 (1421-1430) Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Application : ICC/IF
Granger AJ et al. Elife 2020; 9 Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner.
Gebhardt C et al. Nat Commun 2019; 10 (1):5471 An interhemispheric neural circuit allowing binocular integration in the optic tectum.
DeMarco E et al. J Comp Neurol 2019; (Epub) Neuron types in the zebrafish optic tectum labeled by an id2b transgene.
Application : IHC
Bercier V et al. Mol Neurodegener 2019; 14 (1):27 Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities.
Barth AIM et al. PLoS One 2018; 13 (10):e0204957 Regulation of epithelial migration by epithelial cell adhesion molecule requires its Claudin-7 interaction domain.
Application : ICC/IF
Yao X et al. Dev Cell 2018; 45 (4):526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.
Application : ICC/IF
Zanini D et al. Neuron 2018; (Epub) Proprioceptive Opsin Functions in Drosophila Larval Locomotion.
Birey F et al. Nature 2018; (Epub) Assembly of functionally integrated human forebrain spheroids.
Application : ICC/IF, IHC-Fr
Di Donato V et al. Neuron 2018; (Epub) An Attractive Reelin Gradient Establishes Synaptic Lamination in the Vertebrate Visual System.
Shi Y et al. Nat Med 2018; (Epub) Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.
Application : ICC/IF
Hsu WH et al. Sci Rep 2017; 7 (1):12386 COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring.
Ciarlo C et al. Elife 2017; 6 A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.
Abraira VE et al. Cell 2017; 168 (43102):295-310.e19 The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
Micheva K.D et al. Cellular Imaging 2017; (Epub):149-182 Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons.
Birey F et al. Nature 2017; 545 (7652):54-59 Assembly of functionally integrated human forebrain spheroids.
Application : IHC-Fr
Henson MA et al. Neurobiol Learn Mem 2016; (Epub) Long-term depression-associated signaling is required for an in vitro model of NMDA receptor-dependent synapse pruning.
Application : ICC/IF
Collman F et al. J Neurosci 2015; 35 (14):5792-807 Mapping synapses by conjugate light-electron array tomography.
Matsuyama M et al. Proc Natl Acad Sci U S A 2015; 112 (22):E2947-56 Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain.
Auer TO et al. Elife 2015; 4 (Epub) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.
Saunders A et al. Nature 2015; 521 (7550):31291 A direct GABAergic output from the basal ganglia to frontal cortex.
Application : ICC/IF
Julia L. Semmelhack et al. Elife 2014; 3 A dedicated visual pathway for prey detection in larval zebrafish.
Application : IHC-Wm
Thiele TR et al. Neuron 2014; (Epub) Descending Control of Swim Posture by a Midbrain Nucleus in Zebrafish.
Application : IHC-Wm
Micheva KD et al. Cold Spring Harb Protoc 2010; 2010 (11):pdb.prot5526 Array tomography: imaging stained arrays.
Wyart C et al. Nature 2009; 461 (7262):407-10 Optogenetic dissection of a behavioural module in the vertebrate spinal cord.
Application : IHC
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399