APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human GnRHR Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain of human GnRHR. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gonadotropin releasing hormone receptor , GNRHR1 , GRHR , HH7 , LHRHR , LRHR
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX639094 WB Image
Non-transfected (–) and transfected (+) unboiled PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639094 WB Image
Unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:200 and competitor's antibody (19950-1-AP) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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GTX639094 WB Image
Boiled and unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639094 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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