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Goat Anti-Rabbit IgG antibody, Fab fragment (FITC)

Goat Anti-Rabbit IgG antibody, Fab fragment (FITC). GTX27050

Cat. No. GTX27050

Reference 4

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG Fab

Application

 WB, ICC/IF, Dot, ELISA

Reactivity

 Rabbit
Package
1 mg ($249)
See all Rabbit IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:1000-1:5000
Dot Assay dependent
ELISA 1:10000-1:50000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit IgG whole molecule

Target Isotype

IgG

Purification

IgG fraction
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, papain digestion and chromatographic separation.

Conjugation

Fluorescein isothiocyanate (FITC)
Ratio : 2.0 molecules FITC per Goat IgG Fab molecule.

RRID

AB_378934

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Goat Anti-Rabbit IgG antibody, Fab fragment (FITC). GTX27050

GTX27050 WB Image

WB analysis of rabbit IgG using GTX27050 Goat Anti-Rabbit IgG antibody, Fab fragment (FITC).
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Huang X et al. Mol Pharm 2021; 18 (1) : 377-385 Enhanced Delivery of siRNA to Retinal Ganglion Cells by Intravitreal Lipid Nanoparticles of Positive Charge.
Chang HL et al. Int J Med Sci 2020; 17 (7) : 939-945 The Extracts of Astragalus membranaceus Overcome Tumor Immune Tolerance by Inhibition of Tumor Programmed Cell Death Protein ligand-1 Expression.
Ling Y et al. Arch Toxicol 2020; 94 (3) : 857-871 Triclosan induces zebrafish neurotoxicity by abnormal expression of miR-219 targeting oligodendrocyte differentiation of central nervous system.
Santos MF et al. J Biol Chem 2018; (Epub) VAMP-associated protein-A and oxysterol-binding protein-related protein 3 promote the entry of late endosomes into the nucleoplasmic reticulum.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 249