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HDAC3 antibody [HL1749]

Recombinant antibody KO/KD
Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399
Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399
Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399
Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399
Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399
Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399

Cat. No. GTX637399

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1749

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all HDAC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytosol

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399

GTX637399 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399

GTX637399 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399

GTX637399 IHC-P Image

HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
HDAC3 stained by HDAC3 antibody [HL1749] (GTX637399) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399

GTX637399 IHC-P Image

HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
HDAC3 stained by HDAC3 antibody [HL1749] (GTX637399) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399

GTX637399 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399

GTX637399 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations