Histone H3S10ph (phospho Ser10) antibody

Name: Histone H3S10ph (phospho Ser10) antibody
Brand: GeneTex
SKU: GTX128116
Price: 399.00 USD

Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX128116

Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunoprecipitation (IP). GTX128116

Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

Anti-Histone H3S10ph (phospho Ser10) antibody used in ChIP assay (ChIP assay). GTX128116

Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Whole mount) (IHC-Wm). GTX128116

Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128116

Cat. No. GTX128116

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
Reactivity	Human, Mouse, Rat, Zebrafish, Bovine
Package	100 μl ($399), 25 μl ($169)

See all Histone H3 products

View product citations for antibody GTX128116 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	1:100-1:500
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Positive Control

4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 (100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX128116 IHC-P Image

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128116 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128116 IP Image

Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments.
IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody (GTX128116)
15 % SDS-PAGE
The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX128116 WB Image

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract (untreated)
B. 30 μg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128116 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

GTX128116 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128116 IHC-Wm Image

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100.

GTX128116 WB Image

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128116 ICC/IF Image

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis.
Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

GTX128116 IHC-Wm Image

GTX128116 ICC/IF Image

HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining.

GTX128116 WB Image

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 μg 4T1 whole lysate/extract (untreated)
B. 30 μg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.