GeneTex

  • Country / Location Selection

United States (US)

Human GOT2 protein, His tag (active)

Human GOT2 protein, His tag (active). GTX67022-pro

Cat. No. GTX67022-pro

Application

 Functional Assay

Species

 Human
Package
20 μg ($219)
See all GOT2 protein products

APPLICATION

Application Note

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that converts 1.0 μmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl, 150mM NaCl, 10% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal His-Tag; Length: 424 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Human GOT2 protein, His tag (active). GTX67022-pro

GTX67022-pro Image

3 μg of GTX67022-pro Human GOT2 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Human GOT2 protein, His tag (active) (GTX67022-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Human GOT2 protein, His tag (active) (GTX67022-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Package List Price ($)
$ 219