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Human TGF alpha protein, His tag (active)

Human TGF alpha protein, His tag (active). GTX00100-pro
Human TGF alpha protein, His tag (active). GTX00100-pro
Human TGF alpha protein, His tag (active). GTX00100-pro
Human TGF alpha protein, His tag (active). GTX00100-pro

Cat. No. GTX00100-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($219)
See all TGF alpha protein products

APPLICATION

Application Note

Transforming growth factor alpha   (TGF-α),  a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. To test the effect of TGF-α on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of TGF-α. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of 3T3 cells after incubation with TGF-α for 72h observed by inverted microscope. And TGF-α significantly promoted cell proliferation of 3T3 cells. The ED₅₀ for this effect is typically 0. 6198-8. 210 ng/ml.

Observed MW

15 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu24~Ala98 (NP_001093161.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transforming growth factor alpha , TFGA

Background

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Human TGF alpha protein, His tag (active). GTX00100-pro

GTX00100-pro Functional Assay Image

The dose effect curve of GTX00100-pro Human TGF alpha protein (active) on 3T3 cells. After incubation of Human TGF alpha protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human TGF alpha protein, His tag (active). GTX00100-pro

GTX00100-pro Functional Assay Image

Cell proliferation effect of GTX00100-pro Human TGF alpha protein (active).
(A) Unstimulated 3T3 cells cultured in serum-free DMEM for 72hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1 ng/ml TGF alpha for 72hrs.

Human TGF alpha protein, His tag (active). GTX00100-pro

GTX00100-pro Image

SDS-PAGE analysis of GTX00100-pro Human TGF alpha protein (active).

Human TGF alpha protein, His tag (active). GTX00100-pro

GTX00100-pro Image

WB analysis of GTX00100-pro Human TGF alpha protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 219