GeneTex

  • Country / Location Selection

United States (US)

Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288]

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696
Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696
Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

Cat. No. GTX629696

Reference 11
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT288

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus NA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Influenza A (WSN) infected DF1 cell for 10 hr

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the Internal region of Influenza A virus H1N1 NA (Neuraminidase) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728684

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Neuraminidase , Influenza A Virus NA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] detects Influenza A Virus H1N1 Neuraminidase (NA) protein by western blot analysis.
A. 30 μg DF1 whole cell lysate/extract (untreated)
B. 30 μg whole cell lysate/extract of Influenza A (WSN) infected DF1 cell for 10 hr
10% SDS-PAGE
Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] (GTX629696) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

Influenza A viral lysates were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] (GTX629696) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

The data was published in the journal Viruses in 2019.PMID: 31500389

REFERENCE

Application Reference

Guangwen Wang et al. PLoS Pathog 2022; 18 (4) : e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Xiaoman Liu et al. Nat Commun 2021; 12 (1) : 4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Slaine PD et al. J Virol 2021; Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulation.
Application : WB
Lai Y et al. Front Pharmacol 2021; 12 : 657826 Antiviral Activity of Isoimperatorin Against Influenza A Virus in vitro and its Inhibition of Neuraminidase.
Application : WB
Groenke N et al. Cell Rep 2020; 31 (4) : 107586 Mechanism of Virus Attenuation by Codon Pair Deoptimization.
Application : WB
Show More +
Submit a Reference

REVIEW

Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Cat. No. GTX629696

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629696
Date : Anonymous submitted on 16-Jun-2016
Score :
Application Tested : ICC/IF
Sample : influenza virus A/PR/8/1934 infected MDCK cells
Blocking : 2% BSA in PBS (plus Calcium, Magnesium), 25°C, 1Hr
Fixation : 3%PFA, 2mM MgCl2, 3.6mMCaCl2 in 100mM HEPES pH7.2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Submit a Review
Application Reference
Guangwen Wang et al. PLoS Pathog 2022; 18 (4):e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Xiaoman Liu et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Slaine PD et al. J Virol 2021; Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulation.
Application : WB
Lai Y et al. Front Pharmacol 2021; 12:657826 Antiviral Activity of Isoimperatorin Against Influenza A Virus in vitro and its Inhibition of Neuraminidase.
Application : WB
Groenke N et al. Cell Rep 2020; 31 (4):107586 Mechanism of Virus Attenuation by Codon Pair Deoptimization.
Application : WB
Ha TKQ et al. Biomolecules 2020; 10 (5) Antiviral Activities of Compounds Isolated from Pinus densiflora (Pine Tree) against the Influenza A Virus.
Application : ICC/IF
Omi J et al. Nat Commun 2020; 11 (1):162 The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection.
Application : WB
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Hong JY et al. Antiviral Res 2019; 164:12-22 Highly immunogenic influenza virus-like particles containing B-cell-activating factor (BAFF) for multi-subtype vaccine development.
Application : WB
Guo H et al. PLoS Pathog 2018; 14 (8):e1007233 Kinetic analysis of the influenza A virus HA/NA balance reveals contribution of NA to virus-receptor binding and NA-dependent rolling on receptor-containing surfaces.
Xia C et al. J Virol 2018; (Epub) casein kinase 1α mediates degradation of receptors for type i and type ii *s caused by hemagglutinin of influenza a virus.
Application : WB
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations