Influenza A virus H1N1 NA (Neuraminidase) antibody

Cat. No. GTX125974

Reference 15

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Influenza A virus ( H1N1)
Package	100 μl ($399), 25 μl ($169)

See all Influenza A virus NA1 products

View product citations for antibody GTX125974 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

WSN virus infected DF-1 cells , influenza A virus H1N1-infected H1299

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus H1N1 NA (Neuraminidase) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885621

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Neuraminidase , Influenza A Virus NA

Background

Influenza A subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. There are 16 different HA antigens (H1-H16) and nine different NA antigens (N1-N9) for Influenza A. The extent of infection into host organisms is determined by HA, which interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues.

Research Area

DATA IMAGES

GTX125974 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody (GTX125974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125974 WB Image

Influenza A viral lysates were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody (GTX125974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125974 ICC/IF Image

Influenza A virus H1N1 NA (Neuraminidase) antibody detects Influenza A virus H1N1 NA (Neuraminidase) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus H1N1 NA (Neuraminidase) stained by Influenza A virus H1N1 NA (Neuraminidase) antibody (GTX125974) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX125974 WB Image

Influenza A Virus H1N1 Neuraminidase (NA) antibody detects Influenza A Virus H1N1 Neuraminidase protein by western blot analysis.
A. 5 μg DF-1 whole cell lysate/extract
B. 5 μg whole cell lysate/extract WSN virus infected DF-1 cells
10% SDS-PAGE
Influenza A Virus H1N1 Neuraminidase (NA) antibody (GTX125974) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125974 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody (GTX125974) diluted at 1:1000.

GTX125974 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX125974).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX125974) diluted at 1:100.

GTX125974 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

GTX125974 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

GTX125974 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

REFERENCE

Application Reference

Badruzzaman ATM et al. Vaccines (Basel) 2024; 12 (6) Insect Cell-Based Quadrivalent Seasonal Influenza Virus-like Particles Vaccine Elicits Potent Immune Responses in Mice.

ATM Badruzzaman et al. Vaccines? 2024; 12 (6) : 667 Insect Cell-Based Quadrivalent Seasonal Influenza Virus-like Particles Vaccine Elicits Potent Immune Responses in Mice

YJ Li et al. PLoS Pathog 2024; 20 (5) : e1012279 OTUB1 contributes to the stability and function of Influenza A virus NS2

Li Q et al. Viruses 2024; 16 (5) HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus.

Q Li et al. Viruses 2024; 16 (5) : 702 HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus

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REVIEW

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Application Reference

Badruzzaman ATM et al. Vaccines (Basel) 2024; 12 (6) Insect Cell-Based Quadrivalent Seasonal Influenza Virus-like Particles Vaccine Elicits Potent Immune Responses in Mice.

ATM Badruzzaman et al. Vaccines? 2024; 12 (6):667 Insect Cell-Based Quadrivalent Seasonal Influenza Virus-like Particles Vaccine Elicits Potent Immune Responses in Mice

YJ Li et al. PLoS Pathog 2024; 20 (5):e1012279 OTUB1 contributes to the stability and function of Influenza A virus NS2

Li Q et al. Viruses 2024; 16 (5) HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus.

Q Li et al. Viruses 2024; 16 (5):702 HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus

WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection

Application : ICC/IF

Israa M A Mohamed et al. J Nat Med 2022; 76 (1):152-160 In vitro virucidal activity?of the theaflavin-concentrated tea extract TY-1 against influenza A virus.

Application : WB

Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.