Influenza B virus Nucleoprotein antibody

Cat. No. GTX128539

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA, Sandwich ELISA
Reactivity	Influenza B virus
Package	100 μl ($399), 25 μl ($169)

See all Influenza B virus Nucleoprotein products

View product citations for antibody GTX128539 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:100000
ICC/IF	1:100-1:1000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629882 / GTX629883, Detection : GTX128539 or Capture : GTX128539, Detection : GTX629882

Not tested in other applications.

Positive Control

Influenza B virus (Taiwan B70555) infected medium

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant fragment corresponding to C-terminal region of Influenza B virus NP (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885797

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody (GTX128539) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 WB Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by Western blot analysis.
A. 20 μl non infected medium
B. 20 μl Influenza B virus (Taiwan B70555) infected medium
7.5 % SDS-PAGE
Influenza B Virus NP antibody (GTX128539) dilution: 1:100000

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ICC/IF Image

Influenza B virus Nucleoprotein antibody detects Influenza B virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX128539) diluted at 1:2000.

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: MDCK cells infected with Influenza B virus (Taiwan B70555).
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128539) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128539 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27389476

REFERENCE

Application Reference

Sakai K et al. Sci Rep 2016; 6 : 29430 TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus.

Application : WB

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REVIEW

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Application Reference

Sakai K et al. Sci Rep 2016; 6:29430 TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus.

Application : WB

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Influenza B virus Nucleoprotein antibody

Cat. No. GTX128539

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Positive Control

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX128539 ELISA Image

GTX128539 ELISA Image

GTX128539 WB Image

GTX128539 ELISA Image

GTX128539 ICC/IF Image

GTX128539 ELISA Image

GTX128539 ELISA Image

GTX128539 ICC/IF Image

GTX128539 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.