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Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP)

Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP). GTX628140-01
Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP). GTX628140-01

Cat. No. GTX628140-01

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT881

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Rabbit
Package
250 μl ($199)
See all Rabbit IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Target Isotype

IgG (Heavy chain)

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP). GTX628140-01

GTX628140-01 WB Image

Mouse anti-Rabbit IgG Heavy chain [GT881] antibody detects rabbit heavy chain by Western blot analysis.
A. 2 μg denatured rabbit IgG
10 % SDS-PAGE
Mouse anti-Rabbit IgG Heavy chain [GT881] antibody (GTX628140-01) dilution: 1:10000

Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP). GTX628140-01

GTX628140-01 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-RB] (GTX640415) (1 μg/mL). Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP) (GTX628140-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

Application Reference

Ke Zhang et al. Sci Adv 2021; 7 (6) Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression.
Kim GD et al. Food Sci Nutr 2020; 8 (11) : 5999-6006 Effects of Cudrania tricuspidata on anti-senescence in high glucose-treated endothelial cells via the Akt/p53/p21 pathway.
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SDS
Thimerosal.pdf
Package List Price ($)
$ 199