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NIT2 antibody [2B9]

KO/KD Overexpression
Anti-NIT2 antibody [2B9] used in Flow cytometry (FACS). GTX84036
Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036
Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036
Anti-NIT2 antibody [2B9] used in Western Blot (WB). GTX84036
Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036
Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036
Anti-NIT2 antibody [2B9] used in Western Blot (WB). GTX84036
Anti-NIT2 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84036

Cat. No. GTX84036

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B9

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NIT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitrilase family member 2 , HEL-S-8a

Cellular Localization

Cytoplasm

Background

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Database

Research Area

DATA IMAGES

Anti-NIT2 antibody [2B9] used in Flow cytometry (FACS). GTX84036

GTX84036 FACS Image

FACS analysis of HEK293T cells transfected with either NIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84036 NIT2 antibody [2B9].

Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036

GTX84036 IHC-P Image

IHC-P analysis of liver tissue using GTX84036 NIT2 antibody [2B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036

GTX84036 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84036 NIT2 antibody [2B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [2B9] used in Western Blot (WB). GTX84036

GTX84036 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NIT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84036 NIT2 antibody [2B9].
Loading : 5 ug per lane

Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036

GTX84036 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84036 NIT2 antibody [2B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [2B9] used in IHC (Paraffin sections) (IHC-P). GTX84036

GTX84036 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84036 NIT2 antibody [2B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [2B9] used in Western Blot (WB). GTX84036

GTX84036 WB Image

KO/KD

WB analysis of wild type and NIT2 knock out 293T cell lysate(10μg per lane) using GTX84036 NIT2 antibody [2B9].
Dilution : 1:500

Anti-NIT2 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84036

GTX84036 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NIT2 plasmid using GTX84036 NIT2 antibody [2B9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519