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Nanog antibody, C-term

Anti-Nanog antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89079
Anti-Nanog antibody, C-term used in ELISA (ELISA). GTX89079
Anti-Nanog antibody, C-term used in Western Blot (WB). GTX89079

Cat. No. GTX89079

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Nanog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.05-0.1μg/ml
IHC-P 1-2μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

IHC-P
Human Testis shows nuclear staining in spermatogonia.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89079-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Nanog antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89079

GTX89079 IHC-P Image

IHC-P analysis of human testis using GTX89079 Nanog antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-Nanog antibody, C-term used in ELISA (ELISA). GTX89079

GTX89079 ELISA Image

ELISA analysis of Nanog protein using GTX89079 Nanog antibody, C-term.
Capture : GTX100863 (5μg/ml)
Detection : GTX89079 (5μg/ml)

Anti-Nanog antibody, C-term used in Western Blot (WB). GTX89079

GTX89079 WB Image

WB analysis of HeLa nuclear lysate using GTX89079 Nanog antibody, C-term.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399