RAGE antibody

Name: RAGE antibody
Brand: GeneTex
SKU: GTX23611
Price: 399.00 USD

Anti-RAGE antibody used in Western Blot (WB). GTX23611

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

Cat. No. GTX23611

Reference 20

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat, Frog, Squirrel
Package	100 μl ($399)

See all RAGE products

View product citations for antibody GTX23611 on CiteAb

APPLICATION

Application Note

Recommended Starting Dilutions: For ICC/IF: Use at a dilution of 1:200. For WB: 1:500-1:10000. For IHC-Fr: Use at a concentration of 1-2 ug/ml. For IHC-P: 1:100-1:1000. Optimal dilution / concentration should be determined by the end user.

Positive Control

rat lung , mouse lung

Predict Reactivity

Mouse (94%)| Rat (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_385109

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Advanced Glycosylation End Product-Specific Receptor , Rage , Ager

Research Area

DATA IMAGES

GTX23611 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX23611 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX23611 IHC-P Image

RAGE antibody detects RAGE protein in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
RAGE antibody (GTX23611) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX23611 IHC-P Image

IHC-P analysis of normal mouse lymph node tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX23611 IHC-P Image

IHC-P analysis of normal mouse heart tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX23611 IHC-P Image

IHC-P analysis of normal mouse kidney tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min