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Rat IgG2a isotype control [KLH/G2a-1-1] (FITC)

Cat. No. GTX35056

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 KLH/G2a-1-1

Isotype

 IgG2a

Application

 FACS, ELISA
Package
50 μg ($99)
See all Rat IgG2a isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Product Note

Keyhole limpet hemocyanin (KLH)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keyhole limpet hemocyanin (KLH)

Conjugation

Fluorescein isothiocyanate (FITC) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control for Rat IgG2a kappa , Rat IgG2a negative control , Rat negative control for IgG2a kappa , Rat IgG2a kappa isotype control , Rat isotype control for IgG2a kappa , Negative contr , Rat IgG2a isotype control , Rat IgG2a kappa negative control

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Clone Reference

Marahleh A et al. Front Immunol 2019; 10:2925 TNF-α Directly Enhances Osteocyte RANKL Expression and Promotes Osteoclast Formation.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 99