GeneTex
  • Country / Location Selection

United States (US)

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunoprecipitation (IP). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Flow cytometry (FACS). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

Cat. No. GTX632604

Host

Mouse

Clonality

Monoclonal

Clone Name

1A9

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, EM, Neutralizing/Inhibition, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:500
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
EM Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes.

Sandwich ELISA
Capture : GTX632604, Detection: GTX635654 / GTX135356 / GTX635672 / GTX135386 / GTX135360 / GTX635910 / GTX635911 / GTX635693 / GTX635792 / GTX635793 / GTX635713.
Recommend using GTX400033 (Trident RIPA Lysis Buffer with low SDS) as dilution buffer for diluting samples and antibodies in sandwich ELISA assay if including GTX632604 as capture antibody.

Not tested in other applications.

Product Note

This antibody detects both SARS-CoV spike and SARS-CoV-2 spike proteins (S2 subunit). Based on sequence analysis, this antibody is predicted to recognize S2' subunit. Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2864418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% paraformaldehyde at RT for 30 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag (GTX137114-pro) (28.62-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunoprecipitation (IP). GTX632604

GTX632604 IP Image

Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco-2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) at 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis.
Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone.
Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Blue: Nuclei were counterstained with DAPI.

*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632604

GTX632604 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and Human IgG antibody (HRP) (1:200000) as detection antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604

GTX632604 IHC-P Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of infected human placenta.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Flow cytometry (FACS). GTX632604

GTX632604 FACS Image

FACS analysis of 293T cells infected with recombinant vaccinia virus carrying the Spike gene using GTX632604 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein (GTX135684-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632604

GTX632604 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Red: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604

GTX632604 IHC-P Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green:SARS-CoV-2 (COVID-19) spike protein stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135360 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: Human IgG antibody (HRP) (1:200000 )

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604

GTX632604 IHC-P Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: Goat Anti-Human IgG antibody (HRP) (1:200000)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

The data was published in the 2020 in Sci Rep. PMID: 33318562

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35805887

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35805887

REFERENCE

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] Cat. No. GTX632604

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 3 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Mar-2022
Score :
Application Tested : IHC-P
Sample : Huma skin tissue specimen
Blocking : 0.3% H2O2 meta, RT, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate buffer (ph 6.0)
Primary Antibody : 1:500, 4°C, > 12Hr
Lane Description : Eczema after vaccination. The patient was COVID-19 negative. The signals were accumulated spike proteins which have been produced after mRNA vaccination.
Date : Anonymous submitted on 02-Aug-2020
Score :
Application Tested : IHC-P
Sample Species : SARS-CoV-2
Sample : Human placenta tissue
Fixation : neutral buffered formalin, 25°C, 24Hr
Permeabilization /Antigen retrieval : Citrate buffer pH6, 97°C, 0.3Hr
Primary Antibody : 1:100, 25°C, 0.5Hr
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : IHC-P
Sample Species : SARS-CoV-2
Sample : Human lung tissue
Blocking : 2% BSA + 5% donkey serum + 0.15% PBST, 25°C, 1Hr
Permeabilization /Antigen retrieval : 1% Triton, Sodium Citrate Buffer
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : ICC/IF
Sample Species : SARS-CoV-2
Sample : SARS-CoV-2 FL spike transfected HeLa cells
Primary Antibody : 1μg/ml, 4°C, 16Hr
Date : Mohamed Gadalla submitted on 19-Jun-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV-2
Sample : 293T cells
Amount used : 25 μg
Blocking : 5% non-fat dry milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 17-Jun-2020
Score :
Application Tested : ICC/IF
Sample Species : SARS-CoV-2
Sample : SARS-CoV-2 spike transfected 293T cells
Fixation : 4% PFA, 25°C, 0.5Hr
Primary Antibody : 1:200, 1°C, 25Hr
Date : Anonymous submitted on 31-Mar-2020
Score :
Sample Species : SARS-CoV
Sample : SARS-CoV infected 293T cells
Amount used : 10 μg
Blocking : BSA, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 10-Mar-2020
Score :
Application Tested : ICC/IF
Sample Species : SARS-CoV-2
Sample : BHK-21 transfected with full-length SARS-CoV-2 spike
Fixation : PFA, 0.5Hr
Primary Antibody : 1:2000, 4°C, 4Hr
Figure Note : Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Date : Anonymous submitted on 01-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV, SARS-CoV-2
Sample : SARS-CoV / SARS-CoV-2 infected Caco-2 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Clone Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations