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SMYD3 antibody [HL2464]

Recombinant antibody Orthogonal
Anti-SMYD3 antibody [HL2464] used in IHC (Paraffin sections) (IHC-P). GTX638782
Anti-SMYD3 antibody [HL2464] used in IHC (Paraffin sections) (IHC-P). GTX638782
Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782
Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782
Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782

Cat. No. GTX638782

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2464

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SMYD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SMYD3.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET and MYND domain containing 3 , KMT3E , ZMYND1 , ZNFN3A1 , bA74P14.1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-SMYD3 antibody [HL2464] used in IHC (Paraffin sections) (IHC-P). GTX638782

GTX638782 IHC-P Image

SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMYD3 antibody [HL2464] used in IHC (Paraffin sections) (IHC-P). GTX638782

GTX638782 IHC-P Image

SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782

GTX638782 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782

GTX638782 WB Image

Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-SMYD3 antibody [HL2464] used in Western Blot (WB). GTX638782

GTX638782 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations