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West Nile virus NS3 protein antibody

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

Cat. No. GTX131955

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 West Nile virus
Package
100 μl ($399),
25 μl ($169)
See all West Nile virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

WNV-infected vero cell , WNV NS3-transfected 293T cells , WNV-infected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of West Nile virus NS3 protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

West Nile virus Non-structural protein 3 , West Nile virus NS3 , WNV Non-structural protein 3 , WNV NS3

Research Area

DATA IMAGES

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955).

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:5000.

REFERENCE

Application Reference

Roy P et al. bioRxiv 2023; Motif-VI Loop Acts as a Nucleotide Valve in the West Nile Virus NS3 Helicase.
Tseng AC et al. Viruses 2021; 13 (2) Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication.
Application : WB, ICC/IF
Boys IN et al. Cell Host Microbe 2020; 28 (5) : 712-723.e9 RTP4 Is a Potent IFN-Inducible Anti-flavivirus Effector Engaged in a Host-Virus Arms Race in Bats and Other Mammals.
Application : WB
Submit a Reference

REVIEW

West Nile virus NS3 protein antibody Cat. No. GTX131955

Rating
( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 3.5 based on 2 users reviews)
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:1500, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169