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Wnt10b antibody [HL2370]

Recombinant antibody Overexpression
Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576
Anti-Wnt10b antibody [HL2370] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638576
Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576
Anti-Wnt10b antibody [HL2370] used in IHC (Paraffin sections) (IHC-P). GTX638576
Anti-Wnt10b antibody [HL2370] used in IHC (Paraffin sections) (IHC-P). GTX638576
Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576

Cat. No. GTX638576

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2370

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Wnt10b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Product Note

This antibody is specific for human Wnt10b protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, and Wnt16 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Wnt10b.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 10B , SHFM6 , STHAG8 , WNT-12

Cellular Localization

Secreted, extracellular space, extracellular matrix,Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576

GTX638576 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Wnt10b antibody [HL2370] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638576

GTX638576 ICC/IF Image

Wnt10b antibody [HL2370] detects Wnt10b protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576

GTX638576 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt10b antibody [HL2370] used in IHC (Paraffin sections) (IHC-P). GTX638576

GTX638576 IHC-P Image

Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt10b antibody [HL2370] used in IHC (Paraffin sections) (IHC-P). GTX638576

GTX638576 IHC-P Image

Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt10b antibody [HL2370] used in Western Blot (WB). GTX638576

GTX638576 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169