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p38 MAPK antibody [HL1006]

Recombinant antibody KO/KD
Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797
Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797
Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797
Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797
Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

Cat. No. GTX635797

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1006

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all p38 MAPK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human p38 MAPK recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888577

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

GTX635797 WB Image

KO/KD

Wild-type (WT) and p38 MAPK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

GTX635797 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

GTX635797 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

GTX635797 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [HL1006] used in Western Blot (WB). GTX635797

GTX635797 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

PY Tsai et al. Biochem Pharmacol 2024; 227 : 116423 Aspirin alleviates fibronectin-induced preeclampsia phenotypes in a mouse model and reverses fibronectin-mediated trophoblast invasiveness under hypoxia by regulating ciliogenesis and Akt and MAPK signaling
Application : WB
Reactivity : Human
HM Wang et al. Research Square 2023; Ferulic acid alleviates inflammatory manifestations in atopic dermatitis through modulation of the TRPV1/HMGB1 signaling pathway
Application : WB
Reactivity : Human, Mouse
Xu C et al. Oxid Med Cell Longev 2021; 2021 : 2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations