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p53R2 antibody [HL1400]

Recombinant antibody Orthogonal
Anti-p53R2 antibody [HL1400] used in IHC (Paraffin sections) (IHC-P). GTX636840
Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840
Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840
Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840

Cat. No. GTX636840

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1400

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all p53R2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p53R2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910086

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase regulatory TP53 inducible subunit M2B , MTDPS8A , MTDPS8B , P53R2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-p53R2 antibody [HL1400] used in IHC (Paraffin sections) (IHC-P). GTX636840

GTX636840 IHC-P Image

p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
p53R2 stained by p53R2 antibody [HL1400] (GTX636840) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840

GTX636840 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (GTX636840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840

GTX636840 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (GTX636840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53R2 antibody [HL1400] used in Western Blot (WB). GTX636840

GTX636840 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (GTX636840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations